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When looking at the kinetics of microbial populations, housefly eggs can be incorporated into manure in a high level of addition to provide microbial inoculum for the manure, and allow for potential high microbial degradation rates. The addition of eggs at 30% provides a high level of inoculum when first incorporated; however, this level of egg addition was lowest at the very end of the incubation period (see Table 2). At the end of the 24-h incubation period, the addition of eggs at 30% and 40% was associated with an overall increase of 23.6% in the number of microbial populations (E. faecium), and a decrease of 18.1% (E. coli). The addition of eggs at the end of the incubation may be related to limitations of microbial populations that decrease when the egg addition occurs. Either way, the concept of eggs incorporation into manure at a high level in parallel with the highest level of microbial populations at initial fermentation is a feasible concept to be investigated further.
The concept of using housefly larvae to assist with manure degradation and increase economic value may be further explored by looking at the possibility to include functional components that do not exist in sterile conditions, like biofloc forming bacteria. Poultry litter is a difficult waste to bioconvert due to a high number of additives needed to achieve optimal bioconversion conditions [ 3d9ccd7d82